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| Boonmee, A.; Berthelin, C. Heude; Kingtong, S.; Pauletto, M.; Bernay, B.; Adeline, B.; Suquet, Marc; Sourdaine, P.; Kellner, K.. |
| In order to investigate the mechanisms of final maturation and capacitation of spermatozoa in Pecten maximus, we used a 2D proteomic approach coupled with MALDI-TOF/TOF mass spectrometry (MS) and bioinformatics search against the Pecten database, to set up a reference map of the proteome of spawned spermatozoa, and identified 133 proteins on the basis of the EST database. These proteins are mainly involved in energy production, ion and electron transport (44%), cell movement (22%) and developmental processes (10%). Comparison between proteomes of spermatozoa collected before and after transit through the genital ducts of P. maximus led to the identification of differentially expressed proteins. Most of them are associated with energy metabolism (aconitate... |
| Tipo: Text |
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| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00353/46386/47178.pdf |
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